Cell Culture:Article Title: IL-1b-Bearing NETs: Bridging Inflammation to Early Cirrhosis in Hepatitis B
Article Snippet: Human primary fibroblasts (Fbs) (C-12360, PromoCell, Heidelberg, Germany) were cultured at 37 ◦C with 5% CO2 in low glucose Dulbecco’s Modified Eagle Medium (DMEM, PAN Biotech, Aidenbach, Germany), supplemented with 10% v/v Fetal Bovine Serum (FBS, Capricorn Scientific, Ebsdorfergrund, Germany), 100 U/mL Antibiotic-Antimycotic solution (Biosera, Cholet, France), and 5% v/v MEM Non-Essential Amino Acids Solution (Thermo Fisher Scientific, Waltham, MA, USA).
Article Title: BRAF/MEK inhibition induces cell state transitions boosting immune checkpoint sensitivity in BRAF V600E -mutant glioma.
Article Snippet: 107 Cells were maintained in 50% Neurobasal-A medium without L-Glutamine (10888022; ThermoFisher Scientific, Waltham, MA) and 50% D-MEM/F12 medium (11330-032; ThermoFisher Scientific), supplemented with 0.1mM MEM Non-Essential Amino Acids Solution (11140-050; ThermoFisher Scientific), 1 mM MEM Sodium Pyruvate Solution (11360-070; ThermoFisher Scientific), 1× GlutaMAX-I Supplement (35050-061; ThermoFisher Scientific) and 10 mM HEPES Buffer (15630-080; ThermoFisher Scientific).
Article Title: Methods for culturing cells
Article Snippet: TILs were cultured in either control media or hypotonic conditioning medium with elevated potassium that was supplemented with 2.5% serum supplement (CTSTM Immune Cell SR, Thermo Fisher), 2 mM L-glutamine (Gibco), 2 mM L-glutamax (Gibco), MEM Non-Essential Amino Acids Solution (Gibco), Pen-strep (Gibco), 20 μg/ml FUNGINTM (InvivoGen), Sodium pyruvate (Gibco), and 1 mM of O-Acetyl-L-carnitine hydrochloride (Sigma).
Article Title: Astrocytes control motor neuronal mitochondrial axonal transport deficits in C9ORF72 ALS
Article Snippet: On day 0, the cells were cultured in motor neuron neurotrophic factor (MN-NF) medium, which contains 1X Neurobasal, 1X Antibiotic–Antimycotic, 1X GlutaMAXTM, 1X MEM Non-Essential Amino Acids solution (Gibco Thermo Fisher Scientific), 100 μM beta-mercaptoethanol, 1X B- 27TM supplement, 1X N-2 supplement, RA (1 μM), ascorbic acid (2.5 μM), BDNF (10 ng/ml), recombinant human glial-derived neurotrophic factor (GDNF, 10 ng/ml; R&D Systems Bio- Techne), recombinant human ciliary neurotrophic factor (CNTF, 10 ng/ml; R&D Systems Bio- Techne), and animal-free recombinant human insulin-like growth factor-1 (IGF-1, 10 ng/ml; PeproTech), along with 25 μM uridine/5-fluoro-2′-deoxyuridine (U/FDU).
Article Title: ESC models of autism with copy-number variations reveal cell-type-specific translational vulnerability.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Monoclonal Antibody (3E6) Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11120; RRID: AB_221568 Tuj1 Monoclonal Antibody (Poly18020) Biolegend Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P); RRID: AB_2564645, Cat# 802001 Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11029; RRID: AB_2534088 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11034; RRID: AB_2576217 Chemicals, Peptides, and Recombinant Proteins Dulbecco’s modified Eagle’s medium (DMEM) Thermo Fisher Scientific Cat # 11965092 Glasgow minimum essential medium (GMEM) Sigma Cat# G5154 GibcoTM Fetal Bovine Serum, qualified Thermo Fisher Scientific Cat# 10270-106 KnockOut Serum Replacement (KSR) Thermo Fisher Scientific Cat# 10828028 NeuroCult NSC Basal Medium STEMCELL Technologies Cat# 05700 L-glutamine Thermo Fisher Scientific Cat# 25030149 MEM Non-Essential Amino Acids Solution Thermo Fisher Scientific Cat# 11140076 Adenosine Sigma Cat# A9251 Cytidine Sigma Cat# C4654 Guanosine Sigma Cat# G6752 Thymidine Sigma Cat# T1895 Uridine Sigma Cat# U3750 2-Mercaptoethanol Sigma Cat# M3148 ESGRO Recombinant Mouse LIF Protein Millipore Cat# ESG1107 ACTH (1–24) Bachem Cat# 4059984 G418 Disulfate Aqueous Solution NACALAI TESQUE Cat# 09380-86 B27 supplement Thermo Fisher Scientific Cat# 17504044 Retinoic acid Sigma R2625 Recombinant Human/Murine/Rat BDNF Peprotech Cat# 450-02 Recombinant Human GDNF Peprotech Cat# 450-10 L(+)-Ascorbic Acid, Animal-Free Nakalai tesque Cat# 13048-42 Dibutyryl cAMP sodium salt Sigma Cat# D0627 Taq DNA Polymerase (NEB) NEB Cat# M0273 Flurothyl (bis(2,2,2-trifluoroethyl) ether) Tokyo Chemical Industry (TCI) Cat# B1293 Critical Commercial Assays Lipofectamine LTX Reagent with PLUS Reagent Thermo Fisher Scientific Cat# 15338100 VECTASHIELD Mounting Medium with DAPI Vector Laboratories Cat# H-1200 FluorSave Reagent Sigma Cat# 345789 Fluo-4, AM, cell permeant Thermo Fisher Scientific Cat# F14201 PCR DIG Probe Synthesis Kit Sigma Cat# 11636090910 (Continued on next page) Cell Genomics 5, 100877, June 11, 2025 e1
Article Title: NAMPT and NNMT released via extracellular vesicles and as soluble mediators are distinguished traits of BRAF inhibitor resistance of melanoma cells impacting on the tumor microenvironment
Article Snippet: M14, A375 an SK-MEL-28 BRAFV600E-mutated melanoma cell lines were cultured in RPMI-1640 (Corning Inc., Cat #10-040-CV, Corning, NY, USA) and DMEM (Corning Inc., 10-013-CV) supplemented with 10% (v/v) fetal bovine serum (FBS, Capricorn Scientific, FBS-11 A, #CP21-4466, Ebsdorfergrund, Germany), 1% MEM Non-Essential Amino Acids Solution (Gibco, Thermo Fisher Scientific, #11140035, Segrate, MI, Italy), 1% MEM Vitamin Solution (Gibco, #11120037), 10 mM HEPES Buffer Solution (Capricorn scientific, HEP-B, #CP24-7334), Sodium pyruvate solution 100 mM (Capricorn scientific, NPY-B, #CP23-6474) and 1% Gentamycin (Gibco, #15750-037).
Control:Article Title: IL-1b-Bearing NETs: Bridging Inflammation to Early Cirrhosis in Hepatitis B
Article Snippet: Human primary fibroblasts (Fbs) (C-12360, PromoCell, Heidelberg, Germany) were cultured at 37 ◦C with 5% CO2 in low glucose Dulbecco’s Modified Eagle Medium (DMEM, PAN Biotech, Aidenbach, Germany), supplemented with 10% v/v Fetal Bovine Serum (FBS, Capricorn Scientific, Ebsdorfergrund, Germany), 100 U/mL Antibiotic-Antimycotic solution (Biosera, Cholet, France), and 5% v/v MEM Non-Essential Amino Acids Solution (Thermo Fisher Scientific, Waltham, MA, USA).
Article Title: BRAF/MEK inhibition induces cell state transitions boosting immune checkpoint sensitivity in BRAF V600E -mutant glioma.
Article Snippet: 107 Cells were maintained in 50% Neurobasal-A medium without L-Glutamine (10888022; ThermoFisher Scientific, Waltham, MA) and 50% D-MEM/F12 medium (11330-032; ThermoFisher Scientific), supplemented with 0.1mM MEM Non-Essential Amino Acids Solution (11140-050; ThermoFisher Scientific), 1 mM MEM Sodium Pyruvate Solution (11360-070; ThermoFisher Scientific), 1× GlutaMAX-I Supplement (35050-061; ThermoFisher Scientific) and 10 mM HEPES Buffer (15630-080; ThermoFisher Scientific).
Article Title: Methods for culturing cells
Article Snippet: TILs were cultured in either control media or hypotonic conditioning medium with elevated potassium that was supplemented with 2.5% serum supplement (CTSTM Immune Cell SR, Thermo Fisher), 2 mM L-glutamine (Gibco), 2 mM L-glutamax (Gibco), MEM Non-Essential Amino Acids Solution (Gibco), Pen-strep (Gibco), 20 μg/ml FUNGINTM (InvivoGen), Sodium pyruvate (Gibco), and 1 mM of O-Acetyl-L-carnitine hydrochloride (Sigma).
Article Title: Astrocytes control motor neuronal mitochondrial axonal transport deficits in C9ORF72 ALS
Article Snippet: On day 0, the cells were cultured in motor neuron neurotrophic factor (MN-NF) medium, which contains 1X Neurobasal, 1X Antibiotic–Antimycotic, 1X GlutaMAXTM, 1X MEM Non-Essential Amino Acids solution (Gibco Thermo Fisher Scientific), 100 μM beta-mercaptoethanol, 1X B- 27TM supplement, 1X N-2 supplement, RA (1 μM), ascorbic acid (2.5 μM), BDNF (10 ng/ml), recombinant human glial-derived neurotrophic factor (GDNF, 10 ng/ml; R&D Systems Bio- Techne), recombinant human ciliary neurotrophic factor (CNTF, 10 ng/ml; R&D Systems Bio- Techne), and animal-free recombinant human insulin-like growth factor-1 (IGF-1, 10 ng/ml; PeproTech), along with 25 μM uridine/5-fluoro-2′-deoxyuridine (U/FDU).
Article Title: ESC models of autism with copy-number variations reveal cell-type-specific translational vulnerability.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Monoclonal Antibody (3E6) Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11120; RRID: AB_221568 Tuj1 Monoclonal Antibody (Poly18020) Biolegend Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P); RRID: AB_2564645, Cat# 802001 Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11029; RRID: AB_2534088 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11034; RRID: AB_2576217 Chemicals, Peptides, and Recombinant Proteins Dulbecco’s modified Eagle’s medium (DMEM) Thermo Fisher Scientific Cat # 11965092 Glasgow minimum essential medium (GMEM) Sigma Cat# G5154 GibcoTM Fetal Bovine Serum, qualified Thermo Fisher Scientific Cat# 10270-106 KnockOut Serum Replacement (KSR) Thermo Fisher Scientific Cat# 10828028 NeuroCult NSC Basal Medium STEMCELL Technologies Cat# 05700 L-glutamine Thermo Fisher Scientific Cat# 25030149 MEM Non-Essential Amino Acids Solution Thermo Fisher Scientific Cat# 11140076 Adenosine Sigma Cat# A9251 Cytidine Sigma Cat# C4654 Guanosine Sigma Cat# G6752 Thymidine Sigma Cat# T1895 Uridine Sigma Cat# U3750 2-Mercaptoethanol Sigma Cat# M3148 ESGRO Recombinant Mouse LIF Protein Millipore Cat# ESG1107 ACTH (1–24) Bachem Cat# 4059984 G418 Disulfate Aqueous Solution NACALAI TESQUE Cat# 09380-86 B27 supplement Thermo Fisher Scientific Cat# 17504044 Retinoic acid Sigma R2625 Recombinant Human/Murine/Rat BDNF Peprotech Cat# 450-02 Recombinant Human GDNF Peprotech Cat# 450-10 L(+)-Ascorbic Acid, Animal-Free Nakalai tesque Cat# 13048-42 Dibutyryl cAMP sodium salt Sigma Cat# D0627 Taq DNA Polymerase (NEB) NEB Cat# M0273 Flurothyl (bis(2,2,2-trifluoroethyl) ether) Tokyo Chemical Industry (TCI) Cat# B1293 Critical Commercial Assays Lipofectamine LTX Reagent with PLUS Reagent Thermo Fisher Scientific Cat# 15338100 VECTASHIELD Mounting Medium with DAPI Vector Laboratories Cat# H-1200 FluorSave Reagent Sigma Cat# 345789 Fluo-4, AM, cell permeant Thermo Fisher Scientific Cat# F14201 PCR DIG Probe Synthesis Kit Sigma Cat# 11636090910 (Continued on next page) Cell Genomics 5, 100877, June 11, 2025 e1
Article Title: NAMPT and NNMT released via extracellular vesicles and as soluble mediators are distinguished traits of BRAF inhibitor resistance of melanoma cells impacting on the tumor microenvironment
Article Snippet: M14, A375 an SK-MEL-28 BRAFV600E-mutated melanoma cell lines were cultured in RPMI-1640 (Corning Inc., Cat #10-040-CV, Corning, NY, USA) and DMEM (Corning Inc., 10-013-CV) supplemented with 10% (v/v) fetal bovine serum (FBS, Capricorn Scientific, FBS-11 A, #CP21-4466, Ebsdorfergrund, Germany), 1% MEM Non-Essential Amino Acids Solution (Gibco, Thermo Fisher Scientific, #11140035, Segrate, MI, Italy), 1% MEM Vitamin Solution (Gibco, #11120037), 10 mM HEPES Buffer Solution (Capricorn scientific, HEP-B, #CP24-7334), Sodium pyruvate solution 100 mM (Capricorn scientific, NPY-B, #CP23-6474) and 1% Gentamycin (Gibco, #15750-037).
Modification:Article Title: IL-1b-Bearing NETs: Bridging Inflammation to Early Cirrhosis in Hepatitis B
Article Snippet: Human primary fibroblasts (Fbs) (C-12360, PromoCell, Heidelberg, Germany) were cultured at 37 ◦C with 5% CO2 in low glucose Dulbecco’s Modified Eagle Medium (DMEM, PAN Biotech, Aidenbach, Germany), supplemented with 10% v/v Fetal Bovine Serum (FBS, Capricorn Scientific, Ebsdorfergrund, Germany), 100 U/mL Antibiotic-Antimycotic solution (Biosera, Cholet, France), and 5% v/v MEM Non-Essential Amino Acids Solution (Thermo Fisher Scientific, Waltham, MA, USA).
Article Title: BRAF/MEK inhibition induces cell state transitions boosting immune checkpoint sensitivity in BRAF V600E -mutant glioma.
Article Snippet: 107 Cells were maintained in 50% Neurobasal-A medium without L-Glutamine (10888022; ThermoFisher Scientific, Waltham, MA) and 50% D-MEM/F12 medium (11330-032; ThermoFisher Scientific), supplemented with 0.1mM MEM Non-Essential Amino Acids Solution (11140-050; ThermoFisher Scientific), 1 mM MEM Sodium Pyruvate Solution (11360-070; ThermoFisher Scientific), 1× GlutaMAX-I Supplement (35050-061; ThermoFisher Scientific) and 10 mM HEPES Buffer (15630-080; ThermoFisher Scientific).
Article Title: Methods for culturing cells
Article Snippet: TILs were cultured in either control media or hypotonic conditioning medium with elevated potassium that was supplemented with 2.5% serum supplement (CTSTM Immune Cell SR, Thermo Fisher), 2 mM L-glutamine (Gibco), 2 mM L-glutamax (Gibco), MEM Non-Essential Amino Acids Solution (Gibco), Pen-strep (Gibco), 20 μg/ml FUNGINTM (InvivoGen), Sodium pyruvate (Gibco), and 1 mM of O-Acetyl-L-carnitine hydrochloride (Sigma).
Article Title: Astrocytes control motor neuronal mitochondrial axonal transport deficits in C9ORF72 ALS
Article Snippet: On day 0, the cells were cultured in motor neuron neurotrophic factor (MN-NF) medium, which contains 1X Neurobasal, 1X Antibiotic–Antimycotic, 1X GlutaMAXTM, 1X MEM Non-Essential Amino Acids solution (Gibco Thermo Fisher Scientific), 100 μM beta-mercaptoethanol, 1X B- 27TM supplement, 1X N-2 supplement, RA (1 μM), ascorbic acid (2.5 μM), BDNF (10 ng/ml), recombinant human glial-derived neurotrophic factor (GDNF, 10 ng/ml; R&D Systems Bio- Techne), recombinant human ciliary neurotrophic factor (CNTF, 10 ng/ml; R&D Systems Bio- Techne), and animal-free recombinant human insulin-like growth factor-1 (IGF-1, 10 ng/ml; PeproTech), along with 25 μM uridine/5-fluoro-2′-deoxyuridine (U/FDU).
Article Title: ESC models of autism with copy-number variations reveal cell-type-specific translational vulnerability.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Monoclonal Antibody (3E6) Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11120; RRID: AB_221568 Tuj1 Monoclonal Antibody (Poly18020) Biolegend Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P); RRID: AB_2564645, Cat# 802001 Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11029; RRID: AB_2534088 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11034; RRID: AB_2576217 Chemicals, Peptides, and Recombinant Proteins Dulbecco’s modified Eagle’s medium (DMEM) Thermo Fisher Scientific Cat # 11965092 Glasgow minimum essential medium (GMEM) Sigma Cat# G5154 GibcoTM Fetal Bovine Serum, qualified Thermo Fisher Scientific Cat# 10270-106 KnockOut Serum Replacement (KSR) Thermo Fisher Scientific Cat# 10828028 NeuroCult NSC Basal Medium STEMCELL Technologies Cat# 05700 L-glutamine Thermo Fisher Scientific Cat# 25030149 MEM Non-Essential Amino Acids Solution Thermo Fisher Scientific Cat# 11140076 Adenosine Sigma Cat# A9251 Cytidine Sigma Cat# C4654 Guanosine Sigma Cat# G6752 Thymidine Sigma Cat# T1895 Uridine Sigma Cat# U3750 2-Mercaptoethanol Sigma Cat# M3148 ESGRO Recombinant Mouse LIF Protein Millipore Cat# ESG1107 ACTH (1–24) Bachem Cat# 4059984 G418 Disulfate Aqueous Solution NACALAI TESQUE Cat# 09380-86 B27 supplement Thermo Fisher Scientific Cat# 17504044 Retinoic acid Sigma R2625 Recombinant Human/Murine/Rat BDNF Peprotech Cat# 450-02 Recombinant Human GDNF Peprotech Cat# 450-10 L(+)-Ascorbic Acid, Animal-Free Nakalai tesque Cat# 13048-42 Dibutyryl cAMP sodium salt Sigma Cat# D0627 Taq DNA Polymerase (NEB) NEB Cat# M0273 Flurothyl (bis(2,2,2-trifluoroethyl) ether) Tokyo Chemical Industry (TCI) Cat# B1293 Critical Commercial Assays Lipofectamine LTX Reagent with PLUS Reagent Thermo Fisher Scientific Cat# 15338100 VECTASHIELD Mounting Medium with DAPI Vector Laboratories Cat# H-1200 FluorSave Reagent Sigma Cat# 345789 Fluo-4, AM, cell permeant Thermo Fisher Scientific Cat# F14201 PCR DIG Probe Synthesis Kit Sigma Cat# 11636090910 (Continued on next page) Cell Genomics 5, 100877, June 11, 2025 e1
Article Title: NAMPT and NNMT released via extracellular vesicles and as soluble mediators are distinguished traits of BRAF inhibitor resistance of melanoma cells impacting on the tumor microenvironment
Article Snippet: M14, A375 an SK-MEL-28 BRAFV600E-mutated melanoma cell lines were cultured in RPMI-1640 (Corning Inc., Cat #10-040-CV, Corning, NY, USA) and DMEM (Corning Inc., 10-013-CV) supplemented with 10% (v/v) fetal bovine serum (FBS, Capricorn Scientific, FBS-11 A, #CP21-4466, Ebsdorfergrund, Germany), 1% MEM Non-Essential Amino Acids Solution (Gibco, Thermo Fisher Scientific, #11140035, Segrate, MI, Italy), 1% MEM Vitamin Solution (Gibco, #11120037), 10 mM HEPES Buffer Solution (Capricorn scientific, HEP-B, #CP24-7334), Sodium pyruvate solution 100 mM (Capricorn scientific, NPY-B, #CP23-6474) and 1% Gentamycin (Gibco, #15750-037).
Recombinant:Article Title: IL-1b-Bearing NETs: Bridging Inflammation to Early Cirrhosis in Hepatitis B
Article Snippet: Human primary fibroblasts (Fbs) (C-12360, PromoCell, Heidelberg, Germany) were cultured at 37 ◦C with 5% CO2 in low glucose Dulbecco’s Modified Eagle Medium (DMEM, PAN Biotech, Aidenbach, Germany), supplemented with 10% v/v Fetal Bovine Serum (FBS, Capricorn Scientific, Ebsdorfergrund, Germany), 100 U/mL Antibiotic-Antimycotic solution (Biosera, Cholet, France), and 5% v/v MEM Non-Essential Amino Acids Solution (Thermo Fisher Scientific, Waltham, MA, USA).
Article Title: BRAF/MEK inhibition induces cell state transitions boosting immune checkpoint sensitivity in BRAF V600E -mutant glioma.
Article Snippet: 107 Cells were maintained in 50% Neurobasal-A medium without L-Glutamine (10888022; ThermoFisher Scientific, Waltham, MA) and 50% D-MEM/F12 medium (11330-032; ThermoFisher Scientific), supplemented with 0.1mM MEM Non-Essential Amino Acids Solution (11140-050; ThermoFisher Scientific), 1 mM MEM Sodium Pyruvate Solution (11360-070; ThermoFisher Scientific), 1× GlutaMAX-I Supplement (35050-061; ThermoFisher Scientific) and 10 mM HEPES Buffer (15630-080; ThermoFisher Scientific).
Article Title: Methods for culturing cells
Article Snippet: TILs were cultured in either control media or hypotonic conditioning medium with elevated potassium that was supplemented with 2.5% serum supplement (CTSTM Immune Cell SR, Thermo Fisher), 2 mM L-glutamine (Gibco), 2 mM L-glutamax (Gibco), MEM Non-Essential Amino Acids Solution (Gibco), Pen-strep (Gibco), 20 μg/ml FUNGINTM (InvivoGen), Sodium pyruvate (Gibco), and 1 mM of O-Acetyl-L-carnitine hydrochloride (Sigma).
Article Title: Astrocytes control motor neuronal mitochondrial axonal transport deficits in C9ORF72 ALS
Article Snippet: On day 0, the cells were cultured in motor neuron neurotrophic factor (MN-NF) medium, which contains 1X Neurobasal, 1X Antibiotic–Antimycotic, 1X GlutaMAXTM, 1X MEM Non-Essential Amino Acids solution (Gibco Thermo Fisher Scientific), 100 μM beta-mercaptoethanol, 1X B- 27TM supplement, 1X N-2 supplement, RA (1 μM), ascorbic acid (2.5 μM), BDNF (10 ng/ml), recombinant human glial-derived neurotrophic factor (GDNF, 10 ng/ml; R&D Systems Bio- Techne), recombinant human ciliary neurotrophic factor (CNTF, 10 ng/ml; R&D Systems Bio- Techne), and animal-free recombinant human insulin-like growth factor-1 (IGF-1, 10 ng/ml; PeproTech), along with 25 μM uridine/5-fluoro-2′-deoxyuridine (U/FDU).
Article Title: ESC models of autism with copy-number variations reveal cell-type-specific translational vulnerability.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Monoclonal Antibody (3E6) Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11120; RRID: AB_221568 Tuj1 Monoclonal Antibody (Poly18020) Biolegend Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P); RRID: AB_2564645, Cat# 802001 Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11029; RRID: AB_2534088 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11034; RRID: AB_2576217 Chemicals, Peptides, and Recombinant Proteins Dulbecco’s modified Eagle’s medium (DMEM) Thermo Fisher Scientific Cat # 11965092 Glasgow minimum essential medium (GMEM) Sigma Cat# G5154 GibcoTM Fetal Bovine Serum, qualified Thermo Fisher Scientific Cat# 10270-106 KnockOut Serum Replacement (KSR) Thermo Fisher Scientific Cat# 10828028 NeuroCult NSC Basal Medium STEMCELL Technologies Cat# 05700 L-glutamine Thermo Fisher Scientific Cat# 25030149 MEM Non-Essential Amino Acids Solution Thermo Fisher Scientific Cat# 11140076 Adenosine Sigma Cat# A9251 Cytidine Sigma Cat# C4654 Guanosine Sigma Cat# G6752 Thymidine Sigma Cat# T1895 Uridine Sigma Cat# U3750 2-Mercaptoethanol Sigma Cat# M3148 ESGRO Recombinant Mouse LIF Protein Millipore Cat# ESG1107 ACTH (1–24) Bachem Cat# 4059984 G418 Disulfate Aqueous Solution NACALAI TESQUE Cat# 09380-86 B27 supplement Thermo Fisher Scientific Cat# 17504044 Retinoic acid Sigma R2625 Recombinant Human/Murine/Rat BDNF Peprotech Cat# 450-02 Recombinant Human GDNF Peprotech Cat# 450-10 L(+)-Ascorbic Acid, Animal-Free Nakalai tesque Cat# 13048-42 Dibutyryl cAMP sodium salt Sigma Cat# D0627 Taq DNA Polymerase (NEB) NEB Cat# M0273 Flurothyl (bis(2,2,2-trifluoroethyl) ether) Tokyo Chemical Industry (TCI) Cat# B1293 Critical Commercial Assays Lipofectamine LTX Reagent with PLUS Reagent Thermo Fisher Scientific Cat# 15338100 VECTASHIELD Mounting Medium with DAPI Vector Laboratories Cat# H-1200 FluorSave Reagent Sigma Cat# 345789 Fluo-4, AM, cell permeant Thermo Fisher Scientific Cat# F14201 PCR DIG Probe Synthesis Kit Sigma Cat# 11636090910 (Continued on next page) Cell Genomics 5, 100877, June 11, 2025 e1
Article Title: NAMPT and NNMT released via extracellular vesicles and as soluble mediators are distinguished traits of BRAF inhibitor resistance of melanoma cells impacting on the tumor microenvironment
Article Snippet: M14, A375 an SK-MEL-28 BRAFV600E-mutated melanoma cell lines were cultured in RPMI-1640 (Corning Inc., Cat #10-040-CV, Corning, NY, USA) and DMEM (Corning Inc., 10-013-CV) supplemented with 10% (v/v) fetal bovine serum (FBS, Capricorn Scientific, FBS-11 A, #CP21-4466, Ebsdorfergrund, Germany), 1% MEM Non-Essential Amino Acids Solution (Gibco, Thermo Fisher Scientific, #11140035, Segrate, MI, Italy), 1% MEM Vitamin Solution (Gibco, #11120037), 10 mM HEPES Buffer Solution (Capricorn scientific, HEP-B, #CP24-7334), Sodium pyruvate solution 100 mM (Capricorn scientific, NPY-B, #CP23-6474) and 1% Gentamycin (Gibco, #15750-037).
Purification:Article Title: IL-1b-Bearing NETs: Bridging Inflammation to Early Cirrhosis in Hepatitis B
Article Snippet: Human primary fibroblasts (Fbs) (C-12360, PromoCell, Heidelberg, Germany) were cultured at 37 ◦C with 5% CO2 in low glucose Dulbecco’s Modified Eagle Medium (DMEM, PAN Biotech, Aidenbach, Germany), supplemented with 10% v/v Fetal Bovine Serum (FBS, Capricorn Scientific, Ebsdorfergrund, Germany), 100 U/mL Antibiotic-Antimycotic solution (Biosera, Cholet, France), and 5% v/v MEM Non-Essential Amino Acids Solution (Thermo Fisher Scientific, Waltham, MA, USA).
Article Title: BRAF/MEK inhibition induces cell state transitions boosting immune checkpoint sensitivity in BRAF V600E -mutant glioma.
Article Snippet: 107 Cells were maintained in 50% Neurobasal-A medium without L-Glutamine (10888022; ThermoFisher Scientific, Waltham, MA) and 50% D-MEM/F12 medium (11330-032; ThermoFisher Scientific), supplemented with 0.1mM MEM Non-Essential Amino Acids Solution (11140-050; ThermoFisher Scientific), 1 mM MEM Sodium Pyruvate Solution (11360-070; ThermoFisher Scientific), 1× GlutaMAX-I Supplement (35050-061; ThermoFisher Scientific) and 10 mM HEPES Buffer (15630-080; ThermoFisher Scientific).
Article Title: Methods for culturing cells
Article Snippet: TILs were cultured in either control media or hypotonic conditioning medium with elevated potassium that was supplemented with 2.5% serum supplement (CTSTM Immune Cell SR, Thermo Fisher), 2 mM L-glutamine (Gibco), 2 mM L-glutamax (Gibco), MEM Non-Essential Amino Acids Solution (Gibco), Pen-strep (Gibco), 20 μg/ml FUNGINTM (InvivoGen), Sodium pyruvate (Gibco), and 1 mM of O-Acetyl-L-carnitine hydrochloride (Sigma).
Article Title: Astrocytes control motor neuronal mitochondrial axonal transport deficits in C9ORF72 ALS
Article Snippet: On day 0, the cells were cultured in motor neuron neurotrophic factor (MN-NF) medium, which contains 1X Neurobasal, 1X Antibiotic–Antimycotic, 1X GlutaMAXTM, 1X MEM Non-Essential Amino Acids solution (Gibco Thermo Fisher Scientific), 100 μM beta-mercaptoethanol, 1X B- 27TM supplement, 1X N-2 supplement, RA (1 μM), ascorbic acid (2.5 μM), BDNF (10 ng/ml), recombinant human glial-derived neurotrophic factor (GDNF, 10 ng/ml; R&D Systems Bio- Techne), recombinant human ciliary neurotrophic factor (CNTF, 10 ng/ml; R&D Systems Bio- Techne), and animal-free recombinant human insulin-like growth factor-1 (IGF-1, 10 ng/ml; PeproTech), along with 25 μM uridine/5-fluoro-2′-deoxyuridine (U/FDU).
Article Title: ESC models of autism with copy-number variations reveal cell-type-specific translational vulnerability.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Monoclonal Antibody (3E6) Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11120; RRID: AB_221568 Tuj1 Monoclonal Antibody (Poly18020) Biolegend Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P); RRID: AB_2564645, Cat# 802001 Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11029; RRID: AB_2534088 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11034; RRID: AB_2576217 Chemicals, Peptides, and Recombinant Proteins Dulbecco’s modified Eagle’s medium (DMEM) Thermo Fisher Scientific Cat # 11965092 Glasgow minimum essential medium (GMEM) Sigma Cat# G5154 GibcoTM Fetal Bovine Serum, qualified Thermo Fisher Scientific Cat# 10270-106 KnockOut Serum Replacement (KSR) Thermo Fisher Scientific Cat# 10828028 NeuroCult NSC Basal Medium STEMCELL Technologies Cat# 05700 L-glutamine Thermo Fisher Scientific Cat# 25030149 MEM Non-Essential Amino Acids Solution Thermo Fisher Scientific Cat# 11140076 Adenosine Sigma Cat# A9251 Cytidine Sigma Cat# C4654 Guanosine Sigma Cat# G6752 Thymidine Sigma Cat# T1895 Uridine Sigma Cat# U3750 2-Mercaptoethanol Sigma Cat# M3148 ESGRO Recombinant Mouse LIF Protein Millipore Cat# ESG1107 ACTH (1–24) Bachem Cat# 4059984 G418 Disulfate Aqueous Solution NACALAI TESQUE Cat# 09380-86 B27 supplement Thermo Fisher Scientific Cat# 17504044 Retinoic acid Sigma R2625 Recombinant Human/Murine/Rat BDNF Peprotech Cat# 450-02 Recombinant Human GDNF Peprotech Cat# 450-10 L(+)-Ascorbic Acid, Animal-Free Nakalai tesque Cat# 13048-42 Dibutyryl cAMP sodium salt Sigma Cat# D0627 Taq DNA Polymerase (NEB) NEB Cat# M0273 Flurothyl (bis(2,2,2-trifluoroethyl) ether) Tokyo Chemical Industry (TCI) Cat# B1293 Critical Commercial Assays Lipofectamine LTX Reagent with PLUS Reagent Thermo Fisher Scientific Cat# 15338100 VECTASHIELD Mounting Medium with DAPI Vector Laboratories Cat# H-1200 FluorSave Reagent Sigma Cat# 345789 Fluo-4, AM, cell permeant Thermo Fisher Scientific Cat# F14201 PCR DIG Probe Synthesis Kit Sigma Cat# 11636090910 (Continued on next page) Cell Genomics 5, 100877, June 11, 2025 e1
Article Title: NAMPT and NNMT released via extracellular vesicles and as soluble mediators are distinguished traits of BRAF inhibitor resistance of melanoma cells impacting on the tumor microenvironment
Article Snippet: M14, A375 an SK-MEL-28 BRAFV600E-mutated melanoma cell lines were cultured in RPMI-1640 (Corning Inc., Cat #10-040-CV, Corning, NY, USA) and DMEM (Corning Inc., 10-013-CV) supplemented with 10% (v/v) fetal bovine serum (FBS, Capricorn Scientific, FBS-11 A, #CP21-4466, Ebsdorfergrund, Germany), 1% MEM Non-Essential Amino Acids Solution (Gibco, Thermo Fisher Scientific, #11140035, Segrate, MI, Italy), 1% MEM Vitamin Solution (Gibco, #11120037), 10 mM HEPES Buffer Solution (Capricorn scientific, HEP-B, #CP24-7334), Sodium pyruvate solution 100 mM (Capricorn scientific, NPY-B, #CP23-6474) and 1% Gentamycin (Gibco, #15750-037).
Knock-Out:Article Title: IL-1b-Bearing NETs: Bridging Inflammation to Early Cirrhosis in Hepatitis B
Article Snippet: Human primary fibroblasts (Fbs) (C-12360, PromoCell, Heidelberg, Germany) were cultured at 37 ◦C with 5% CO2 in low glucose Dulbecco’s Modified Eagle Medium (DMEM, PAN Biotech, Aidenbach, Germany), supplemented with 10% v/v Fetal Bovine Serum (FBS, Capricorn Scientific, Ebsdorfergrund, Germany), 100 U/mL Antibiotic-Antimycotic solution (Biosera, Cholet, France), and 5% v/v MEM Non-Essential Amino Acids Solution (Thermo Fisher Scientific, Waltham, MA, USA).
Article Title: BRAF/MEK inhibition induces cell state transitions boosting immune checkpoint sensitivity in BRAF V600E -mutant glioma.
Article Snippet: 107 Cells were maintained in 50% Neurobasal-A medium without L-Glutamine (10888022; ThermoFisher Scientific, Waltham, MA) and 50% D-MEM/F12 medium (11330-032; ThermoFisher Scientific), supplemented with 0.1mM MEM Non-Essential Amino Acids Solution (11140-050; ThermoFisher Scientific), 1 mM MEM Sodium Pyruvate Solution (11360-070; ThermoFisher Scientific), 1× GlutaMAX-I Supplement (35050-061; ThermoFisher Scientific) and 10 mM HEPES Buffer (15630-080; ThermoFisher Scientific).
Article Title: Methods for culturing cells
Article Snippet: TILs were cultured in either control media or hypotonic conditioning medium with elevated potassium that was supplemented with 2.5% serum supplement (CTSTM Immune Cell SR, Thermo Fisher), 2 mM L-glutamine (Gibco), 2 mM L-glutamax (Gibco), MEM Non-Essential Amino Acids Solution (Gibco), Pen-strep (Gibco), 20 μg/ml FUNGINTM (InvivoGen), Sodium pyruvate (Gibco), and 1 mM of O-Acetyl-L-carnitine hydrochloride (Sigma).
Article Title: Astrocytes control motor neuronal mitochondrial axonal transport deficits in C9ORF72 ALS
Article Snippet: On day 0, the cells were cultured in motor neuron neurotrophic factor (MN-NF) medium, which contains 1X Neurobasal, 1X Antibiotic–Antimycotic, 1X GlutaMAXTM, 1X MEM Non-Essential Amino Acids solution (Gibco Thermo Fisher Scientific), 100 μM beta-mercaptoethanol, 1X B- 27TM supplement, 1X N-2 supplement, RA (1 μM), ascorbic acid (2.5 μM), BDNF (10 ng/ml), recombinant human glial-derived neurotrophic factor (GDNF, 10 ng/ml; R&D Systems Bio- Techne), recombinant human ciliary neurotrophic factor (CNTF, 10 ng/ml; R&D Systems Bio- Techne), and animal-free recombinant human insulin-like growth factor-1 (IGF-1, 10 ng/ml; PeproTech), along with 25 μM uridine/5-fluoro-2′-deoxyuridine (U/FDU).
Article Title: ESC models of autism with copy-number variations reveal cell-type-specific translational vulnerability.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Monoclonal Antibody (3E6) Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11120; RRID: AB_221568 Tuj1 Monoclonal Antibody (Poly18020) Biolegend Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P); RRID: AB_2564645, Cat# 802001 Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11029; RRID: AB_2534088 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11034; RRID: AB_2576217 Chemicals, Peptides, and Recombinant Proteins Dulbecco’s modified Eagle’s medium (DMEM) Thermo Fisher Scientific Cat # 11965092 Glasgow minimum essential medium (GMEM) Sigma Cat# G5154 GibcoTM Fetal Bovine Serum, qualified Thermo Fisher Scientific Cat# 10270-106 KnockOut Serum Replacement (KSR) Thermo Fisher Scientific Cat# 10828028 NeuroCult NSC Basal Medium STEMCELL Technologies Cat# 05700 L-glutamine Thermo Fisher Scientific Cat# 25030149 MEM Non-Essential Amino Acids Solution Thermo Fisher Scientific Cat# 11140076 Adenosine Sigma Cat# A9251 Cytidine Sigma Cat# C4654 Guanosine Sigma Cat# G6752 Thymidine Sigma Cat# T1895 Uridine Sigma Cat# U3750 2-Mercaptoethanol Sigma Cat# M3148 ESGRO Recombinant Mouse LIF Protein Millipore Cat# ESG1107 ACTH (1–24) Bachem Cat# 4059984 G418 Disulfate Aqueous Solution NACALAI TESQUE Cat# 09380-86 B27 supplement Thermo Fisher Scientific Cat# 17504044 Retinoic acid Sigma R2625 Recombinant Human/Murine/Rat BDNF Peprotech Cat# 450-02 Recombinant Human GDNF Peprotech Cat# 450-10 L(+)-Ascorbic Acid, Animal-Free Nakalai tesque Cat# 13048-42 Dibutyryl cAMP sodium salt Sigma Cat# D0627 Taq DNA Polymerase (NEB) NEB Cat# M0273 Flurothyl (bis(2,2,2-trifluoroethyl) ether) Tokyo Chemical Industry (TCI) Cat# B1293 Critical Commercial Assays Lipofectamine LTX Reagent with PLUS Reagent Thermo Fisher Scientific Cat# 15338100 VECTASHIELD Mounting Medium with DAPI Vector Laboratories Cat# H-1200 FluorSave Reagent Sigma Cat# 345789 Fluo-4, AM, cell permeant Thermo Fisher Scientific Cat# F14201 PCR DIG Probe Synthesis Kit Sigma Cat# 11636090910 (Continued on next page) Cell Genomics 5, 100877, June 11, 2025 e1
Article Title: NAMPT and NNMT released via extracellular vesicles and as soluble mediators are distinguished traits of BRAF inhibitor resistance of melanoma cells impacting on the tumor microenvironment
Article Snippet: M14, A375 an SK-MEL-28 BRAFV600E-mutated melanoma cell lines were cultured in RPMI-1640 (Corning Inc., Cat #10-040-CV, Corning, NY, USA) and DMEM (Corning Inc., 10-013-CV) supplemented with 10% (v/v) fetal bovine serum (FBS, Capricorn Scientific, FBS-11 A, #CP21-4466, Ebsdorfergrund, Germany), 1% MEM Non-Essential Amino Acids Solution (Gibco, Thermo Fisher Scientific, #11140035, Segrate, MI, Italy), 1% MEM Vitamin Solution (Gibco, #11120037), 10 mM HEPES Buffer Solution (Capricorn scientific, HEP-B, #CP24-7334), Sodium pyruvate solution 100 mM (Capricorn scientific, NPY-B, #CP23-6474) and 1% Gentamycin (Gibco, #15750-037).
Fluorsave:Article Title: IL-1b-Bearing NETs: Bridging Inflammation to Early Cirrhosis in Hepatitis B
Article Snippet: Human primary fibroblasts (Fbs) (C-12360, PromoCell, Heidelberg, Germany) were cultured at 37 ◦C with 5% CO2 in low glucose Dulbecco’s Modified Eagle Medium (DMEM, PAN Biotech, Aidenbach, Germany), supplemented with 10% v/v Fetal Bovine Serum (FBS, Capricorn Scientific, Ebsdorfergrund, Germany), 100 U/mL Antibiotic-Antimycotic solution (Biosera, Cholet, France), and 5% v/v MEM Non-Essential Amino Acids Solution (Thermo Fisher Scientific, Waltham, MA, USA).
Article Title: BRAF/MEK inhibition induces cell state transitions boosting immune checkpoint sensitivity in BRAF V600E -mutant glioma.
Article Snippet: 107 Cells were maintained in 50% Neurobasal-A medium without L-Glutamine (10888022; ThermoFisher Scientific, Waltham, MA) and 50% D-MEM/F12 medium (11330-032; ThermoFisher Scientific), supplemented with 0.1mM MEM Non-Essential Amino Acids Solution (11140-050; ThermoFisher Scientific), 1 mM MEM Sodium Pyruvate Solution (11360-070; ThermoFisher Scientific), 1× GlutaMAX-I Supplement (35050-061; ThermoFisher Scientific) and 10 mM HEPES Buffer (15630-080; ThermoFisher Scientific).
Article Title: Methods for culturing cells
Article Snippet: TILs were cultured in either control media or hypotonic conditioning medium with elevated potassium that was supplemented with 2.5% serum supplement (CTSTM Immune Cell SR, Thermo Fisher), 2 mM L-glutamine (Gibco), 2 mM L-glutamax (Gibco), MEM Non-Essential Amino Acids Solution (Gibco), Pen-strep (Gibco), 20 μg/ml FUNGINTM (InvivoGen), Sodium pyruvate (Gibco), and 1 mM of O-Acetyl-L-carnitine hydrochloride (Sigma).
Article Title: Astrocytes control motor neuronal mitochondrial axonal transport deficits in C9ORF72 ALS
Article Snippet: On day 0, the cells were cultured in motor neuron neurotrophic factor (MN-NF) medium, which contains 1X Neurobasal, 1X Antibiotic–Antimycotic, 1X GlutaMAXTM, 1X MEM Non-Essential Amino Acids solution (Gibco Thermo Fisher Scientific), 100 μM beta-mercaptoethanol, 1X B- 27TM supplement, 1X N-2 supplement, RA (1 μM), ascorbic acid (2.5 μM), BDNF (10 ng/ml), recombinant human glial-derived neurotrophic factor (GDNF, 10 ng/ml; R&D Systems Bio- Techne), recombinant human ciliary neurotrophic factor (CNTF, 10 ng/ml; R&D Systems Bio- Techne), and animal-free recombinant human insulin-like growth factor-1 (IGF-1, 10 ng/ml; PeproTech), along with 25 μM uridine/5-fluoro-2′-deoxyuridine (U/FDU).
Article Title: ESC models of autism with copy-number variations reveal cell-type-specific translational vulnerability.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Monoclonal Antibody (3E6) Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11120; RRID: AB_221568 Tuj1 Monoclonal Antibody (Poly18020) Biolegend Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P); RRID: AB_2564645, Cat# 802001 Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11029; RRID: AB_2534088 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11034; RRID: AB_2576217 Chemicals, Peptides, and Recombinant Proteins Dulbecco’s modified Eagle’s medium (DMEM) Thermo Fisher Scientific Cat # 11965092 Glasgow minimum essential medium (GMEM) Sigma Cat# G5154 GibcoTM Fetal Bovine Serum, qualified Thermo Fisher Scientific Cat# 10270-106 KnockOut Serum Replacement (KSR) Thermo Fisher Scientific Cat# 10828028 NeuroCult NSC Basal Medium STEMCELL Technologies Cat# 05700 L-glutamine Thermo Fisher Scientific Cat# 25030149 MEM Non-Essential Amino Acids Solution Thermo Fisher Scientific Cat# 11140076 Adenosine Sigma Cat# A9251 Cytidine Sigma Cat# C4654 Guanosine Sigma Cat# G6752 Thymidine Sigma Cat# T1895 Uridine Sigma Cat# U3750 2-Mercaptoethanol Sigma Cat# M3148 ESGRO Recombinant Mouse LIF Protein Millipore Cat# ESG1107 ACTH (1–24) Bachem Cat# 4059984 G418 Disulfate Aqueous Solution NACALAI TESQUE Cat# 09380-86 B27 supplement Thermo Fisher Scientific Cat# 17504044 Retinoic acid Sigma R2625 Recombinant Human/Murine/Rat BDNF Peprotech Cat# 450-02 Recombinant Human GDNF Peprotech Cat# 450-10 L(+)-Ascorbic Acid, Animal-Free Nakalai tesque Cat# 13048-42 Dibutyryl cAMP sodium salt Sigma Cat# D0627 Taq DNA Polymerase (NEB) NEB Cat# M0273 Flurothyl (bis(2,2,2-trifluoroethyl) ether) Tokyo Chemical Industry (TCI) Cat# B1293 Critical Commercial Assays Lipofectamine LTX Reagent with PLUS Reagent Thermo Fisher Scientific Cat# 15338100 VECTASHIELD Mounting Medium with DAPI Vector Laboratories Cat# H-1200 FluorSave Reagent Sigma Cat# 345789 Fluo-4, AM, cell permeant Thermo Fisher Scientific Cat# F14201 PCR DIG Probe Synthesis Kit Sigma Cat# 11636090910 (Continued on next page) Cell Genomics 5, 100877, June 11, 2025 e1
Article Title: NAMPT and NNMT released via extracellular vesicles and as soluble mediators are distinguished traits of BRAF inhibitor resistance of melanoma cells impacting on the tumor microenvironment
Article Snippet: M14, A375 an SK-MEL-28 BRAFV600E-mutated melanoma cell lines were cultured in RPMI-1640 (Corning Inc., Cat #10-040-CV, Corning, NY, USA) and DMEM (Corning Inc., 10-013-CV) supplemented with 10% (v/v) fetal bovine serum (FBS, Capricorn Scientific, FBS-11 A, #CP21-4466, Ebsdorfergrund, Germany), 1% MEM Non-Essential Amino Acids Solution (Gibco, Thermo Fisher Scientific, #11140035, Segrate, MI, Italy), 1% MEM Vitamin Solution (Gibco, #11120037), 10 mM HEPES Buffer Solution (Capricorn scientific, HEP-B, #CP24-7334), Sodium pyruvate solution 100 mM (Capricorn scientific, NPY-B, #CP23-6474) and 1% Gentamycin (Gibco, #15750-037).
Polymerase Chain Reaction:Article Title: IL-1b-Bearing NETs: Bridging Inflammation to Early Cirrhosis in Hepatitis B
Article Snippet: Human primary fibroblasts (Fbs) (C-12360, PromoCell, Heidelberg, Germany) were cultured at 37 ◦C with 5% CO2 in low glucose Dulbecco’s Modified Eagle Medium (DMEM, PAN Biotech, Aidenbach, Germany), supplemented with 10% v/v Fetal Bovine Serum (FBS, Capricorn Scientific, Ebsdorfergrund, Germany), 100 U/mL Antibiotic-Antimycotic solution (Biosera, Cholet, France), and 5% v/v MEM Non-Essential Amino Acids Solution (Thermo Fisher Scientific, Waltham, MA, USA).
Article Title: BRAF/MEK inhibition induces cell state transitions boosting immune checkpoint sensitivity in BRAF V600E -mutant glioma.
Article Snippet: 107 Cells were maintained in 50% Neurobasal-A medium without L-Glutamine (10888022; ThermoFisher Scientific, Waltham, MA) and 50% D-MEM/F12 medium (11330-032; ThermoFisher Scientific), supplemented with 0.1mM MEM Non-Essential Amino Acids Solution (11140-050; ThermoFisher Scientific), 1 mM MEM Sodium Pyruvate Solution (11360-070; ThermoFisher Scientific), 1× GlutaMAX-I Supplement (35050-061; ThermoFisher Scientific) and 10 mM HEPES Buffer (15630-080; ThermoFisher Scientific).
Article Title: Methods for culturing cells
Article Snippet: TILs were cultured in either control media or hypotonic conditioning medium with elevated potassium that was supplemented with 2.5% serum supplement (CTSTM Immune Cell SR, Thermo Fisher), 2 mM L-glutamine (Gibco), 2 mM L-glutamax (Gibco), MEM Non-Essential Amino Acids Solution (Gibco), Pen-strep (Gibco), 20 μg/ml FUNGINTM (InvivoGen), Sodium pyruvate (Gibco), and 1 mM of O-Acetyl-L-carnitine hydrochloride (Sigma).
Article Title: Astrocytes control motor neuronal mitochondrial axonal transport deficits in C9ORF72 ALS
Article Snippet: On day 0, the cells were cultured in motor neuron neurotrophic factor (MN-NF) medium, which contains 1X Neurobasal, 1X Antibiotic–Antimycotic, 1X GlutaMAXTM, 1X MEM Non-Essential Amino Acids solution (Gibco Thermo Fisher Scientific), 100 μM beta-mercaptoethanol, 1X B- 27TM supplement, 1X N-2 supplement, RA (1 μM), ascorbic acid (2.5 μM), BDNF (10 ng/ml), recombinant human glial-derived neurotrophic factor (GDNF, 10 ng/ml; R&D Systems Bio- Techne), recombinant human ciliary neurotrophic factor (CNTF, 10 ng/ml; R&D Systems Bio- Techne), and animal-free recombinant human insulin-like growth factor-1 (IGF-1, 10 ng/ml; PeproTech), along with 25 μM uridine/5-fluoro-2′-deoxyuridine (U/FDU).
Article Title: ESC models of autism with copy-number variations reveal cell-type-specific translational vulnerability.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Monoclonal Antibody (3E6) Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11120; RRID: AB_221568 Tuj1 Monoclonal Antibody (Poly18020) Biolegend Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P); RRID: AB_2564645, Cat# 802001 Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11029; RRID: AB_2534088 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Thermo Fisher Scientific Cat# A-11034; RRID: AB_2576217 Chemicals, Peptides, and Recombinant Proteins Dulbecco’s modified Eagle’s medium (DMEM) Thermo Fisher Scientific Cat # 11965092 Glasgow minimum essential medium (GMEM) Sigma Cat# G5154 GibcoTM Fetal Bovine Serum, qualified Thermo Fisher Scientific Cat# 10270-106 KnockOut Serum Replacement (KSR) Thermo Fisher Scientific Cat# 10828028 NeuroCult NSC Basal Medium STEMCELL Technologies Cat# 05700 L-glutamine Thermo Fisher Scientific Cat# 25030149 MEM Non-Essential Amino Acids Solution Thermo Fisher Scientific Cat# 11140076 Adenosine Sigma Cat# A9251 Cytidine Sigma Cat# C4654 Guanosine Sigma Cat# G6752 Thymidine Sigma Cat# T1895 Uridine Sigma Cat# U3750 2-Mercaptoethanol Sigma Cat# M3148 ESGRO Recombinant Mouse LIF Protein Millipore Cat# ESG1107 ACTH (1–24) Bachem Cat# 4059984 G418 Disulfate Aqueous Solution NACALAI TESQUE Cat# 09380-86 B27 supplement Thermo Fisher Scientific Cat# 17504044 Retinoic acid Sigma R2625 Recombinant Human/Murine/Rat BDNF Peprotech Cat# 450-02 Recombinant Human GDNF Peprotech Cat# 450-10 L(+)-Ascorbic Acid, Animal-Free Nakalai tesque Cat# 13048-42 Dibutyryl cAMP sodium salt Sigma Cat# D0627 Taq DNA Polymerase (NEB) NEB Cat# M0273 Flurothyl (bis(2,2,2-trifluoroethyl) ether) Tokyo Chemical Industry (TCI) Cat# B1293 Critical Commercial Assays Lipofectamine LTX Reagent with PLUS Reagent Thermo Fisher Scientific Cat# 15338100 VECTASHIELD Mounting Medium with DAPI Vector Laboratories Cat# H-1200 FluorSave Reagent Sigma Cat# 345789 Fluo-4, AM, cell permeant Thermo Fisher Scientific Cat# F14201 PCR DIG Probe Synthesis Kit Sigma Cat# 11636090910 (Continued on next page) Cell Genomics 5, 100877, June 11, 2025 e1
Article Title: NAMPT and NNMT released via extracellular vesicles and as soluble mediators are distinguished traits of BRAF inhibitor resistance of melanoma cells impacting on the tumor microenvironment
Article Snippet: M14, A375 an SK-MEL-28 BRAFV600E-mutated melanoma cell lines were cultured in RPMI-1640 (Corning Inc., Cat #10-040-CV, Corning, NY, USA) and DMEM (Corning Inc., 10-013-CV) supplemented with 10% (v/v) fetal bovine serum (FBS, Capricorn Scientific, FBS-11 A, #CP21-4466, Ebsdorfergrund, Germany), 1% MEM Non-Essential Amino Acids Solution (Gibco, Thermo Fisher Scientific, #11140035, Segrate, MI, Italy), 1% MEM Vitamin Solution (Gibco, #11120037), 10 mM HEPES Buffer Solution (Capricorn scientific, HEP-B, #CP24-7334), Sodium pyruvate solution 100 mM (Capricorn scientific, NPY-B, #CP23-6474) and 1% Gentamycin (Gibco, #15750-037).
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